Abstrak

Komposisi media Sabouraud Dextrose Agar (SDA) terdiri dari dekstrosa, pepton dan agar sebagai pemadat media. Dekstrosa mengandung karbohidrat dan pepton mengandung protein sebagai unsur penting dalam pertumbuhan Aspergillus flavus. Jagung (Zea mays) dan kacang tanah (Arachis hypogea) dipilih sebagai media alternatif dikarenakan memiliki komposisi karbohidrat dan protein yang diperlukan oleh Aspergillus flavus. Secara eksperimen akan dilakukan variasi komposisi media alternatif berbahan dasar Zea mays dan Arachis hypogea dengan empat variasi media. Aspergillus flavus ditanam dengan teknik single dot dan diinkubasi selama 5 hari pada suhu 37^oC. Hasil penelitian menunjukkan bahwa media alternatif B (15,75g Zea mays dan 5,34g Arachis hypogea) memberikan diameter koloni dan panjang hifa yang tidak berbeda signifikan terhadap media SDA dengan nilai p value secara berturut-turut adalah 0,188 dan 0,790. Pemanfaatan bahan alami dalam pembuatan media dapat menjadi alternatif teknologi green value chain di bidang mikrobiologi lingkungan.

Kata kunci: media alternatif, diameter koloni, panjang hifa, Aspergillus flavus.

Abstract

Sabouraud Dextrose Agar SDA media composition consists of dextrose, peptone and agar as a media compactor. Dextrose contains carbohydrate and peptone contains protein as an important element in the growth of Aspergillus flavus. Corn (Zea mays) and peanuts (Arachis hypogea) were chosen as alternative media because they have the composition of carbohydrates and proteins needed by Aspergillus flavus. Experiments will be carried out variations in the composition of alternative media made from corn and peanuts with four media variations. Aspergillus flavus is planted by single dot technique and incubated for 5 days at 37oC. The results showed that alternative media B (15,75g of corn and 5.34g of peanut) gave a colony diameter and hyphae length that were not significantly different from the SDA media with p values of respectively 0.188 and 0.790.

Keywords: alternative media, colony diameter, hyphae length, Aspergillus flavus.

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