Modifikasi Media Jagung (Zea mays) dan Kacang Tanah (Arachis hypogea) sebagai Media Pertumbuhan Aspergillus flavus

NOVI FITRIA, FUJI SETIAWATI

Sari


Abstrak

Komposisi media Sabouraud Dextrose Agar (SDA) terdiri dari dekstrosa, pepton dan agar sebagai pemadat media. Dekstrosa mengandung karbohidrat dan pepton mengandung protein sebagai unsur penting dalam pertumbuhan Aspergillus flavus. Jagung (Zea mays) dan kacang tanah (Arachis hypogea) dipilih sebagai media alternatif dikarenakan memiliki komposisi karbohidrat dan protein yang diperlukan oleh Aspergillus flavus. Secara eksperimen akan dilakukan variasi komposisi media alternatif berbahan dasar Zea mays dan Arachis hypogea dengan empat variasi media. Aspergillus flavus ditanam dengan teknik single dot dan diinkubasi selama 5 hari pada suhu 37oC. Hasil penelitian menunjukkan bahwa media alternatif B (15,75g Zea mays dan 5,34g Arachis hypogea) memberikan diameter koloni dan panjang hifa yang tidak berbeda signifikan terhadap media SDA dengan nilai p value secara berturut-turut adalah 0,188 dan 0,790. Pemanfaatan bahan alami dalam pembuatan media dapat menjadi alternatif teknologi green value chain di bidang mikrobiologi lingkungan.

Kata kunci: media alternatif, diameter koloni, panjang hifa, Aspergillus flavus.


Abstract

Sabouraud Dextrose Agar SDA media composition consists of dextrose, peptone and agar as a media compactor. Dextrose contains carbohydrate and peptone contains protein as an important element in the growth of Aspergillus flavus. Corn (Zea mays) and peanuts (Arachis hypogea) were chosen as alternative media because they have the composition of carbohydrates and proteins needed by Aspergillus flavus. Experiments will be carried out variations in the composition of alternative media made from corn and peanuts with four media variations. Aspergillus flavus is planted by single dot technique and incubated for 5 days at 37oC. The results showed that alternative media B (15,75g of corn and 5.34g of peanut) gave a colony diameter and hyphae length that were not significantly different from the SDA media with p values of respectively 0.188 and 0.790.

Keywords: alternative media, colony diameter, hyphae length, Aspergillus flavus.




Teks Lengkap:

PDF

Referensi


Abrunhosa, L., Paterson, Z.R.R.M., Kozakiewicz, N., Lima, A Venancio. (2001). Mycotoxin production from fungi isolated from grapes. Letters in Applied Microbiology, 32 (4), 240-242.

Adisarwanto, T. (2000). Meningkatkan Produksi Kacang Tanah di Lahan Sawah & Lahan Kering. Penebar Swadaya. Jakarta.

Ernawati, A., Adipati, Y.C. (2017). Identifikasi Jamur Pada Biji Jagung (Zea Mays Busuk dan Segar yang dijual di Pasar Baru Borong Makassar, Prosiding Seminar Biology for Life. ISBN 978-602-72245-2-0.

Cotty P.J & Jaime-Garcia R. (2007). Influences of Climate on Aflatoxin Producing Fungi and Aflatoxin Contamination. Int J. Food Microbiology, 119 (1-2), 109-115.

Guinea, J. Pelaez, T., Alcala, L., Bouza, E. (2005). Evaluation of Czapeck Agar and Sabouraud Dextrose Agar for the Culture of Airborne Aspergillus conidia, Diagnostic Microbiology and Infectious Disease, 53, 333-334.

Julius P. Sserumaga, Alejandro Ortega-Beltran, John M. Wagacha., Charity K. Mutegi., Ranajit Bandyopadhyay. (2020). Aflatoxin-producing Fungi Associated with Pre-harvest Maize Contamination in Uganda. Internasional Journal of Food Microbiology 313,108376.

Lan, H., Wu, L., Sun, R., Yang. K., Liu, Y., Wu, J., Geng, L., Huang, C., Wang, S. (2018). Investigation of Aspergillus falvus in Animal Virulence, Toxicon, 145, 40-47.

Nesci, A. & Etchevery, M. (2002). Aspergillus Section Flavi Populations From Field Maize in Argentina, Letter Applied Microbiology, 34, 343-348.

Omeiza, G.K., Kabir, J., Kwaga, J.K.P., Kwanashie, C.N., Mwanza, M., Ngoma, L. (2018). A Risk Assessment Study of the Occurrence and Distribution of Aflatoxigen Aspergillus flavus and Aflatoxin B1 in Diary Cattle feeds in a Central Northern State, Nigeria, Toxicology Reports, 5, 846-856.

Paul W. Kachapulula., Juliet Akello, RanajitBandyopadhyay., Peter J. Cotty. (2017). Aspergillus Section Flavi Community Structure in Zambia Influences Aflatoxin Contamination of Maize and Groundnut. Internasional Journal of Food Microbiology, 261, 49-56.

Probst, C., Bandyopadhyay, Cotty, J.P. (2014). Diversity of Aflatoxin-producing Fungi and Their Impact on Food Safety in sub-saharan Africa, Internasional Journal of Food Microbiology, 174, 113-122.

Reverberi, M., A.A. Fabbri, S., Zjalic, A., Ricelli, F., Punneli, C., Fanelli. (2005). Antioxidants Enzymes Stimulations in Aspergillus Parasiticus by Lentinula Edodes Inhibits Aflatoxin Production, Applied Microbiology Biotechnology, 69 (2), 207-215.

Ruban, V.V., Anbukkarasi, M., Anand, T., Thomas, P.A., Geraldine, P. (2019). Oxidative Stress in Corneal Tissue in Experimental Keratitis duet to Aspergillus flavus : Effect of Topical Voriconazole Therapy, Biocatalysis and Agricultural Biotechnology, 21, 101323.

Samson, R.A., Hoekstra, E.S., Van Oorschot, C.A. (1999). Introducing To Food-Borde Fungi. Centralbureau Voor Schimmelcultures.




DOI: https://doi.org/10.26760/rekalingkungan.v8i1.57-66

Refbacks

  • Saat ini tidak ada refbacks.



Terindeks:

 

Statistik Pengunjung